Fig 3. Effect of inhibitors of HDAC on cytosolic Ca²⁺ in Picea wilsonii pollen tubes.

(A) Fluo-3/AM staining of pollen tubes cultured in standard medium for 24 h. (B) Fluo-3/AM staining of pollen tubes incubated in standard medium supplemented with 0.2% DMSO for 24 h. (C) The fluorescence intensity was analyzed using ImageJ from the base to the tip of the (A) pollen tube. The slope coefficient became larger. (D) The fluorescence intensity was analyzed using ImageJ from the base to the tip of the (B) pollen tube. The slope coefficient became larger. (E) Fluo-3/AM staining of pollen tubes incubated in medium supplemented with 0.5 μM TSA for 24 h. (F) Fluo-3/AM staining of pollen tubes incubated in medium supplemented with 0.5 mM NaB for 24 h. (G) The fluorescence intensity was analyzed using ImageJ from the base to the tip of the (E) pollen tube. The slope coefficient was largely unchanged. (H) The fluorescence intensity was analyzed using ImageJ from the base to the tip of the (F) pollen tube. The slope coefficient was largely unchanged. Bar = 50 μm.