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. 2015 Dec 28;10(12):e0144133. doi: 10.1371/journal.pone.0144133

Fig 5. HREC do not upregulate CX3CL1 in response to CD40 ligation or TNF-α and do not secrete TNF-α after CD40 ligation.

Fig 5

(A–D) HREC and HAEC transduced with empty retroviral vector or retroviral vector that encodes wt CD40 were incubated with or without multimeric CD154 for 24 h. (A) Expression of membrane-bound CX3CL1 was assessed by flow cytometry. (B) Expression of ICAM-1 on HREC was assessed by flow cytometry. (C) CX3CL1 concentrations in supernatants were determined by ELISA. (D) CCL2 concentrations in supernatants from HREC were determined by ELISA. (E–F) Untransduced HAEC and HREC were incubated with TNF-α as indicated for 24 h. Expression of membrane-bound (E) and soluble CX3CL1 (F) were assessed by flow cytometry and ELISA respectively. Results are shown as mean ± SEM and are representative of 4 experiments. *** p < 0.001.