Fig 3. Setdb2 is systemically upregulapted in a murine model of IAV infection.

Setdb2 ^LacZ reporter mice were inoculated with PBS or IAV (1 x 10⁴ PFU) to determine the proportion of inflammatory populations expressing lacZ in the lungs (A), blood (B), and spleen (C) on day 0 and 4 post-infection. Leukocytes were phenotypically characterized by the following surface markers: neutrophils (Ly6G^high CD11b⁺ CD11c^-), inflammatory monocytes (Ly6C^high CD11b⁺ CD11c^- CCR2⁺), alveolar Mϕ (F4/80⁺ CD11c⁺ CD11b^low/- MHC-II^low/int), tissue Mϕ (F4/80⁺ CD11b⁺ CD11c^- MHC-II^high), conventional DCs (MHC-II^high CD11b⁺ CD11c⁺), NK cells (NK1.1⁺ CD3^-), B cells (B220⁺ CD4^- CD8^-), CD4⁺ T cells (CD4⁺ CD3⁺), and CD8⁺ T cells (CD8⁺ CD3⁺). Data are mean ± SEM (n = 6–12 mice) from 2–3 independent experiments. *p<0.05; **p<0.01; ***p<0.001.