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. 2016 Jan;147(1):77–93. doi: 10.1085/jgp.201511448

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© 2016 Zheng et al.

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Figure 1. — Chemical principles behind the formation of bSUMs. (A) Surface functionalization through chemical oxidization and bioconjugation. (B) Binding of transmembrane proteins via active ligands on the surface of the beads. (C) Reconstitution of a lipid bilayer around the anchored membrane proteins on the beads. (D) Electrical recordings from reconstituted channels in bSUMs by using a planar electrode, which would be equivalent to a cell-attached patch. (E) After the formation of gigaohm seal on a planar electrode, the vesicle of a bSUM usually ruptures outside the sealed area and leads to the opening from the top partition to the intravesicular side of the bSUM, similar to an excised patch (inside-out mode).