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. 2016 Jan;147(1):77–93. doi: 10.1085/jgp.201511448

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© 2016 Zheng et al.

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Figure 8. — bSUMs of varying dimension and lipid composition. Electrical recordings from KvAP bSUMs of 5 (A), 10 (B) and 20 µm (C) in diameter, or made of different lipid mixture, 3:1 (wt/wt) POPE/POPG (D), DOPC (E), 95:5 (wt/wt) DOPC/cholesterol (F). Test pulses depolarizing from 0 to 160 mV at 40-mV steps were introduced every 2 min. The holding potential was −80 mV. The standard intracellular solution (80 mM NaCl, 200 mM KCl, 1 mM MgCl₂, 2 mM CaCl₂, and 10 mM HEPES/KOH, pH 7.4) and extracellular solution (10 mM NaCl, 200 mM KCl, 60 mM KF, 20 mM EGTA, and 10 mM HEPES/KOH, pH 7.4) were used.