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. 2015 Dec 28;10(12):e0145677. doi: 10.1371/journal.pone.0145677

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© 2015 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — MSCs-TetR/Amelx at 3 passages were cultured in the growth medium (Con) or osteogenic induction medium (Os) in the presence (+) or absence (-) of Dox in 24-well plates. (a) ALP activity on day 7 and 14 was examined by ALP staining (bars: 100 μm). (b) Mineralized nodule formation on day 21, 28 and 35 was detected by von Kossa staining. (c, d): Calcium deposition was determined by Alizarin Red S staining on day 7, 14 and 21 (c) and the staining intensity was quantitatively analyzed (d). Significant differences (*P<0.01: ANOVA with Tukey’s multiple comparison test: n = 9) are shown.