| Cycling Step | Temperature | Time | # Cycles |
| Enzyme activation | 95 °C | 10 min | 1 |
| Denaturation | 94 °C | 30 sec | 40 |
| Annealing/extension | 60 °C | 1 min * | |
| Hold | 98 °C | 10 min | 1 |
| Hold | 4 °C | Forever | 1 |
| * Adjust ramp rate settings to 2-2.5 °C/sec. Use a heated lid set to 105 °C and set the sample volume to 40 μl |
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