Cycling Step | Temperature | Time | # Cycles |
Enzyme activation | 95 °C | 10 min | 1 |
Denaturation | 94 °C | 30 sec | 40 |
Annealing/extension | 60 °C | 1 min * | |
Hold | 98 °C | 10 min | 1 |
Hold | 4 °C | Forever | 1 |
* Adjust ramp rate settings to 2-2.5 °C/sec. Use a heated lid set to 105 °C and set the sample volume to 40 μl |