E
Freeze thaw stability, -20 °C, 1 cycle
QC level [ng/ml]	2	4	20	40
	92.5	86.2	90.2	94.3
89.8	90.5	85.4	104
94.7	88.6	93.4	104
101	89.2	93.7	96.3
% of nominal concentration	94.5	88.6	90.7	99.7
Imprecision [%CV]	4.8	1.8	3.9	5.1
Freeze thaw stability, -20 °C, 2 cycles
QC level [ng/ml]	2	4	20	40
	99.1	97.6	Failed	85.3
93.1	88.1	93.4	92.0
94.9	91.5	85.8	93.9
Failed	90.5	86.8	85.4
% of nominal concentration	95.7	91.9	88.7	89.2
Imprecision [%CV]	3.1	4.0	4.1	4.5
Freeze thaw stability, -20 °C, 3 cycles
QC level [ng/ml]	2	4	20	40
	95.7	Failed	86.0	93.3
95.5	87.4	85.0	91.3
90.5	89.1	86.0	86.0
96.9	85.7	90.2	Failed
% of nominal concentration	94.7	87.4	86.8	90.2
Imprecision [%CV]	2.8	1.7	2.3	3.8