| Extraction Pump | Analytical (Elution) Pump | ||||||
| Time [min] | Water + 0.1% formic acid | Acetonitrile | Flow rate [µl/min] | Time [min] | Water + 0.1% formic acid | Acetonitrile | Flow rate [µl/min] |
| 0 | 70 | 30 | 5,000 | 0 | 13 | 87 | 1,000 |
| 1 | 70 | 30 | 5,000 | 2 | 2 | 98 | 1,200 |
| 1.1 | 2 | 98 | 100 | 3.5 | 2 | 98 | 1,200 |
| 3 | 2 | 98 | 100 | 3.6 | 13 | 87 | 1,000 |
| 3.1 | 20 | 80 | 2,000 | 4.6 | 13 | 87 | 1,000 |
| 4 | 70 | 30 | 5,000 | ||||
| 4.6 | 70 | 30 | 5,000 |
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