Reagent	Stock Concentration	MW	Final Volume	Mass Added
(M)	(g/mol)	(ml)	(g)
Tris/HCl, pH 7.4	2	157.56	250	78.8
Tris/HCl, pH 7.4	1	157.56	250	39.39
KCL	1	74.55	500	37.28
Tris Base	1	121.14	100	12.11
EDTA, pH 8	0.5	416.2	100 ml (in 1 M Tris Base)	20.81
EGTA, pH 7.2	0.1	380.35	100 (in 1 M Tris Base)	3.801