Reagent	Volume (µl)	Final concentration
ROX-<c* tc*> at 100 µM	10	10 µM (1x)
<c>-RQ at 100 µM	13	13 µM (1.3x)
10x TAE with 125 mM Mg2+	10	1x TAE with 12.5 mM Mg2+
H2O	67	--
Total volume	100	10 µM (1x)