Reagent	Volume (µl)	Final concentration
H2O	514	-
10x TAE with 125 mM Mg2+	60	1x TAE with 12.5 mM Mg2+
PolyT at 300 µM	2	1 µM
ReporterC at 10 µM	9	150 nM (3x)
10% SDS	9	0.15%
<tc c> at 5 µM	6	50 nM (1x)
Total volume	600	-