Reagent	Volume (µl)	Final concentration
H2O	407.2	-
10x TAE with 125 mM Mg2+	52.8	12.5 mM Mg2+
polyT at 300 µM	2	1 µM
ReporterC at 10 µM	9	150 nM (3x)
<i tc> at 10 µM	6	100 nM (2x)
<c tb> at 10 µM	6	100 nM (2x)
<b tr> at 10 µM	6	100 nM (2x)
<tr r> at 10 µM	6	100 nM (2x)
10% SDS	9	0.15%
JoinAB at 1 µM	45	75 nM (1.5x)
ForkBC at 1 µM	45	75 nM (1.5x)
<ta a> at 10 µM	3	50 nM (1x)
<tb b> at 10 µM	3	50 nM (1x)
Total volume	600	-