| Synthesized gates | Plasmid-derived gates | ||||
| Description | Cost | Join gates | Fork gates | ||
| PAGE purified long strand (100 nt; served as the bottom strands of a gate) | ~$75 | Description | Cost | Description | Cost |
| PAGE purified short strand (~30 nt, served as top strands of a gate) | ~$185 | Gate template | ~$100 | Gate template | ~$100 |
| Total | ~$260 | Plasmid extraction kit | ~$26 | Plasmid extraction kit | ~$26 |
| Restriction enzyme (PvuII-HF) | ~$11 | Restriction enzyme (PvuII-HF) | ~$11 | ||
| Nicking enzyme (Nt.BsrDI, Join gates) | ~$29 | Nicking enzyme (Nt.BstNBI, Fork gates) | ~$62 | ||
| Total | ~$166 | Total | ~$199 |
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