| Synthesized gates | Plasmid-derived gates | ||
| Processing | Processing time | Processing | Processing time |
| Annealing | 1 hr | Cloning | 5 hr |
| PAGE purification | 2 hr | Plasmid extraction | 2 hr |
| Total | 3 hr | Two steps of enzyme digestion | 0.5 hr |
| Ethanol precipitation | 1 hr | ||
| Total | 8.5 hr |
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