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In vitro characterization of the cellular distribution of human TENM4 mutants. Localization of wild-type (wt) and mutated forms of TENM4 in Oli-neu cells. Wild-type TENM4 as well as the control allele G7933A (c.7933G>A) showed a homogeneous membrane distribution. In contrast, the mutated forms C4100A (c.4100C>A), G4324A (c.4324 G>A) and G3412A (c.3412G>A) showed clustered localization at the membrane. TENM4 is in red; DAPI nuclear dye is in blue.
