Figure 2.

3-MA enhances liver toxicity in WT mice and KI mice whereas rapamycin is protective against liver injury. Chronic alcohol fed WT, KO and KI mice were treated with saline, 3-MA or rapamycin as mentioned in the legend to Figure 1. Liver slides were prepared for H&E staining and the extent of liver injury including steatosis and necrosis was observed under a light microscope. Arrowheads point to lipid droplets, and the arrow points to necroinflammation.