Figure 3.

Regulation of gal promoters by CRP complex (CCC). (A) Model of interactions between CCC (yellow) at the activating site (−40.5) and RNAP (brown) at the −10 and −35 elements of P1 (+1). P2 is located at −5 The αNTD and αCTD of RNAP contact both subunits of Class II promoters at CCC as shown in Figure 4 (adapted from [25]); (B) RNAs made typically from P1 and P2 promoters in the absence (−) and presence (+) of CCC as analyzed by gel electrophoresis. The concentrations of cAMP and CRP are 100 μM and 50 nM, respectively. RNAI is a control RNA in the plasmid (reproduced with permission from Elsevier [14]).