Fig. 2.
Growth curve and frequency of excisants in MRSA N315 grown in TSB. Bacteria were grown at 37 °C as described (closed diamonds) and sampled after 3, 24 and 72 h for DNA extraction and qPCR analysis. The open columns represent the ratio of excised versus chromosomes copies at each sampling time. Error bars represent the average ± SD of three independent measurements performed in three independent cultures