FIG. 5.

Real time q-PCR curves of extracted nucleic acids from spiked HPV samples. (a) The positive control reaction has 5 × 10⁸ copies of stock HPV plasmid. The starting number of HPV plasmids in each sample is shown. For each sample, one twentieth of the extracted amount of HPV plasmid is used in the real time PCR mixture. The number of copies used in real time PCR is shown in brackets. (b) The calculated recovered number of HPV plasmids is plotted against the actual input number of HPV plasmids. The R² value is 0.999 and one error bar denotes 1 standard deviation (s.d.).