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. 2015 Aug 28;14(6):945–956. doi: 10.1111/acel.12380

Figure 1.

Figure 1

eRapa alters gene expression in immune cells resulting in preferential reduction in memory T lymphocytes. (A) Scatterplots of normalized gene expression values (Log2 scale) in sorted CD4+ PD‐1+, CD4+ PD‐1−, CD8+ PD‐1+, and CD8+ PD‐1− T cells comparing cells from eRapa‐fed 22‐month‐old male BL6 mice (RAPA, Y‐axis) versus Eudragit control (CTRL, X‐axis) mice (mean, n = 3 mice/group). The numbers of genes upregulated (green) or downregulated (red) by more than twofold in eRapa versus CTRL are indicated. Representative genes are annotated. (B) Numbers of total spleen cells, CD3+, CD4+, and CD8+ T cells from young (8 months old) and aged (24–25 months old) male and female C57BL/6 mice on Eudragit (CTRL) or eRapa (RAPA) chow for 6 months (n = 10‐15 mice/group). (C) Frequency of CD62L+ cells in CD4+ and CD8+ splenic T cells from young (8 months old) and aged (24–25 months old) male and female C57BL/6 mice on Eudragit (CTRL) or eRapa (RAPA) chow for 6 months (n = 10–15 mice/group). All error bars represent standard error of mean (SEM).