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. 2015 Oct 1;14(6):1075–1084. doi: 10.1111/acel.12401

Figure 5.

Figure 5

Protection from oxidative stress in AC5 KO mice contributed to the enhanced exercise capacity. (A) Relative MnSOD mRNA levels were increased in the skeletal muscle in systemic AC5 KO and AC5 SKM KO; mating AC5 KO mice with heterozygous MnSOD KO abolished increased MnSOD mRNA levels in AC5 KO; n = 6. (B) MnSOD expression was increased in the skeletal muscle from AC5 KO compared with WT mice; mating AC5 KO mice with heterozygous MnSOD KO abolished increased MnSOD in AC5 KO n = 4. (C) Paraquat reduced exercise capacity significantly in WT, but not in AC5 KO. *P < 0.01 vs. WT treated with vehicle using one‐way ANOVA ; n = 6. (D) Mating AC5 KO mice with heterozygous MnSOD KO abolished enhanced exercise capacity in AC5 KO mice. (E) 8‐OHdG staining of gastrocnemius muscle sections after paraquat treatment showed less staining in AC5 KO. *P < 0.01 vs. WT and **P < 0.05 vs. AC5 KO using one‐way ANOVA; n = 5. Results are expressed as the mean ± SEM. AC, adenylyl cyclase; KO, knock out.