Figure 4.

Hypothalamic inflammation in GH‐treated Ames mice. Brain sections of 18‐month‐old mice were analyzed for hypothalamic microglia and TNF‐α. (A) Representative images showing immunostaining in the MBH subregion of control and Ames dwarf mice, and in Ames mice that had been treated either with saline or GH. Scale bars: 100 μm (far left); 20 μm (right side panels).(B) Numbers of cells immunoreactive for Iba‐1, or TNF‐α in the hypothalamic mediobasal region (across the confocal microscopic field of serial sections) from indicated male mice (n = 4 to 6/group); error bars show SEM *P < 0.05 versus control.