Figure 6.

Western blot analysis of tumour necrosis factor‐α (TNF‐α), tumour growth factor‐β (TGF‐β) and MyoD in crude extracts of intrinsic laryngeal muscles (ILM) from C57BL/10 and mdx mice at 2 and 20 months of age. Bands indicate Western blot of TNF‐α, TGF‐β and MyoD and the same blot reprobed for GAPDH as a loading control. Graphs represent the level of each indicated protein expressed in arbitrary units normalized to GAPDH levels. No differences were seen in the levels of the markers between control and dystrophic muscles, at the ages studied.