Table 1.

Early RAPA treatment fails to rescue myelination

Antibody	Control V	CKO V	Control RAPA	CKO RAPA
pS6 (ser240/244)	100.00 ± 1.96	221.20 ± 19.57	32.81 ± 4.77	49.80 ± 5.67
MBP	100.00 ± 0.48	34.63 ± 5.78a	33.23 ± 9.95a	34.80 ± 7.29a
MAG	100.00 ± 3.39	43.20 ± 8.31a	59.58 ± 9.96a	67.49 ± 6.67
CNPase	100.00 ± 3.44	13.48 ± 3.55a	49.81 ± 16.93	31.38 ± 10.48a
GFAP	100.00 ± 13.08	227.30 ± 23.62a	87.88 ± 11.42	158.80 ± 20.44
PGP9.5	100.00 ± 2.37	95.61 ± 14.08	83.20 ± 7.77	81.28 ± 2.98

Expression of myelin proteins in P17 cortical extracts from mice treated with 0.1 mg/kg rapamycin or vehicle from P3 to P17. Data represent mean ± SEM, n = 3–6 animals per group. CKO, conditional knockout; V, vehicle; RAPA, rapamycin; MBP, myelin basic protein; MAG, myelin‐associated glycoprotein; CNPase, 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase; GFAP, glial fibrillary acidic protein; ANOVA, analysis of variance.

^a P < 0.05 by one‐way ANOVA versus control vehicle.