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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Fungal Genet Biol. 2015 Jun 23;82:9–21. doi: 10.1016/j.fgb.2015.06.003

Figure 5. Virulence- and cell wall-associated phenotypes of C. neoformans insertional mutants identified by AIM-Seq.

Figure 5

(a). Indicated strains were normalized, serially diluted, and incubated on YPD medium with the addition of indicated cell wall perturbing agents or at the indicated temperatures. (b). Strains were incubated in capsule-inducing conditions (CO2- independent medium, 37°C) with shaking for 72 hours. Capsule was assessed by India ink counterstaining, followed by imaging (630x). (c). Melanin production was assessed by the appearance of brown pigment in colonies incubated on Niger seed agar for 2-3 days at the indicated temperatures.