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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Fungal Genet Biol. 2015 Jun 23;82:9–21. doi: 10.1016/j.fgb.2015.06.003

Figure 6. Cell wall chitin and chitosan exposure as assessed by staining of C. neoformans insertional mutants identified by AIM-Seq.

Figure 6

Strains were incubated in CO2- independent medium at 37°C for 18 hours prior to staining and imaging. Exposure of cell wall components was assessed by staining with (a) CFW and WGA for chitin, and (b) eosin Y for chitosan. Stained cells were imaged by fluorescent microscopy (630x).