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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Fungal Genet Biol. 2015 Jun 23;82:9–21. doi: 10.1016/j.fgb.2015.06.003

Figure 8. Vacuolar staining of WHM27/cnag_02676ΔT-DNA.

Figure 8

Strains were incubated in YPD liquid media at 30°C for 18 hours prior to staining and imaging. Cells were stained with the endocytosis marker, FM4-64, for 10 minutes on ice, followed by washing and incubation at room temperature for 30 minutes. Stained cells were imaged by fluorescent microscopy (630x). Black arrows indicate FM4-64 staining of vacuolar membranes.