Table 5.
AMT strains phenotypically validated in independent mutant strains
| Phenotypesb | ||||||
|---|---|---|---|---|---|---|
|
| ||||||
| AMT Strain ID |
Independent Disruption |
Gene ID | Annotation | pH 8 | 1.5 M NaCl |
0.06% SDS |
| WHM4 | -- | CNAG_05142 | alpha-1,3- mannosyltransferase |
+ | ± | − |
| -- | WHM4-SKEa | + | ± | − | ||
|
| ||||||
| WHM11 | -- | CNAG_02793 | kynurenine 3- monooxygenase |
± | + | − |
| -- | WHM11-SKE | ± | + | − | ||
|
| ||||||
| WHM21 | -- | CNAG 03409, CNAG_03410 |
Skn7, cytoplasmic protein |
+ | ± | ± |
| -- | skn7Δ | + | ± | + | ||
|
| ||||||
| WHM24 | -- | CNAG_05218 | adenylyl cyclase- associated protein |
± | − | − |
| -- | aca1Δ | ± | − | − | ||
|
| ||||||
| WHM27 | -- | CNAG_02676 | conserved hypothetical protein |
+ | − | − |
| -- | WHM27-SKE | + | − | − | ||
a
Generated by disruption with original T-DNA insert in the H99 MATα background
b
+: robust growth, ±: moderate growth/sensitivity, −: no growth