Figure 6.
DHEA attenuates histamine‐induced paracellular leak in human vascular endothelial cell line EA.hy926. A: Transendothelial resistance (TER) and B: HRP flux in DHEA‐treated human vascular endothelial cell line (EA.hy926) after histamine stimulation. Confluent (>100 Ω/cm2) vascular endothelial cells treated with vehicle or DHEA (100 nM) were stimulated with histamine (100 μM) for 30 min, and TER and HRP flux were determined. Data are representative of 5 wells per treatment group from two independent experiments. Individual circles represent an individual well. Column represents mean ± SD from n = 2 experiments. *P < 0.05, **P < 0.01, ***P < 0.005.