Figure 2.

T cell subsets in mice lacking S100A4 expression. A: Subsets of thymocytes in S100a4^Gfp/Gfp or S100a4^+/+ mice were identified by flow cytometry. Relative numbers of CD4⁻ CD8⁻ DN, CD4⁺ CD8⁺ DP, CD4⁺ CD8⁻ SP, and CD4⁻ CD8⁺ SP cells in thymocytes isolated from S100a4^Gfp/Gfp or S100a4^+/+ mice. Mean ± SD. n = 5 mice per group. *P < 0.05. B: Subsets of spleen cells in S100a4^−/− or S100a4^+/+ mice were identified by flow cytometry. Relative numbers of B220⁺ B cells as well as CD3⁺, CD4⁺, or CD8⁺ T cells in spleen cells isolated from S100a4^Gfp/Gfp or S100a4^+/+ mice. Mean ± SD. n = 5 mice per group. C: Subsets of lymph node cells in S100a4^−/− or S100a4^+/+ mice were identified by flow cytometry. Relative numbers of B220⁺ B cells as well as CD3⁺, CD4⁺, or CD8⁺ T cells in lymph node cells isolated from S100a4^Gfp/Gfp or S100a4^+/+ mice. Mean ± SD. n = 5 mice per group. D: Natural FOXP3⁺ T regulatory cells were identified by flow cytometry. Relative numbers of FOXP3⁺ T cells within splenic CD4⁺ CD3⁺ T cells isolated from S100a4^Gfp/Gfp or S100a4^+/+ mice. Mean ± S n = 5 mice per group. *P < 0.05.