Figure 3.

Memory T cells express S100A4. A: Western blot analysis of S100A4 expression in purified naive or memory CD4⁺ T cells. Positive control for S100A4 expression included lysate from NIH‐3T3 fibroblasts. B: Subsets of central (T_CM) and effector (T_EM) memory CD8⁺ T cells were identified in the spleen of S100a4^Gfp/Gfp or S100a4^+/+ mice by flow cytometry. Relative numbers of CD44^low CD62L^high naive T cells, CD44^high CD62L^high T_CM and CD44^high CD62L^low T_EM within CD3⁺ CD8⁺ T cells isolated from S100a4^Gfp/Gfp or S100a4^+/+ mice. Mean ± SD. n = 5 mice per group. C: Subsets of memory CD4⁺ T cells were identified in the spleen of S100a4^Gfp/Gfp or S100a4^+/+ mice. Relative numbers of CD44^low CD62L^high naive T cells and CD44^high CD62L^low memory T cells within CD3⁺ CD4⁺ T cells isolated from S100a4^Gfp/Gfp or S100a4^+/+ mice. Mean ± SD. n = 5 mice per group. D: S100A4 expression in naive, central (T_CM) and effector (T_EM) memory CD8⁺ T cells. Relative numbers of S100A4‐expressing cells (% GFP⁺ cells) within CD44^low CD62L^high naive, CD44^high CD62L^high T_CM, or CD44^high CD62L^low T_EM CD3⁺ CD8⁺ T cells were determine by flow cytometry. Mean ± S n = 5 mice per group. *P < 0.05. E: S100A4 expression in naive and memory CD4⁺ T cells. Relative numbers of S100A4‐expressing cells (% GFP⁺ cells) within CD44^low CD62L^high naive or CD44^high CD62L^low T_EM CD3⁺ CD4⁺ T cells were determine by flow cytometry. Mean ± SD. n = 5 mice per group. *P < 0.05.