Figure 6.

S100A4 expression in CD4⁺ T cells is not required for the motility of T cells and the development of autoimmune inflammation. A: The capacity of purified S100s4^Gfp/Gfp or S100a4^+/+ memory CD4⁺ T cells to migrate toward gradients of CXCL10 concentration was carried out in a transwell migration assay (see Methods). Mean ± SD. n = 5 culture replicates. Data are representative of four independent experiments. B: Percent of initial weight in Rag2^−/− mice adoptively transferred with CD45RB^high CD4⁺ T cells isolated from S100s4^Gfp/Gfp or S100a4^+/+ mice. n = 10 mice per experimental group. C: Percent of initial weight (upper panel) or EAE clinical score (lower panel) in Rag2^−/− mice adoptively transferred with MOG‐specific CD4⁺ T cells isolated from MOG‐immunized S100s4^Gfp/Gfp or S100a4^+/+ mice. n = 10–12 mice per experimental group. D: Rag2^−/− mice adoptively transferred with MOG‐specific CD4⁺ T cells isolated from MOG‐immunized S100s4^Gfp/Gfp or S100a4^+/+ mice, were harvested and CD4⁺ CD3⁺ T cells in the brains were analyzed by flow cytometry to determine their relative number in cellular isolates, as well as to detect among them the frequency of GFP‐expressing cells. Mean ± S n = 5 mice for the group transferred with S100a4^+/+ CD4⁺ T cells and n = 6 mice for the group transferred with S100a4^Gfp/Gfp. E. Rag2^−/− mice adoptively transferred as in D. CD4⁺ T cells in the brain were analyzed by flow cytometry to detect cells expressing IL‐17A and IFN‐γ. Mean ± SD. n = 5 mice for the group transferred with S100a4^+/+ CD4⁺ T cells and n = 6 mice for the group transferred with S100a4^Gfp/Gfp.