Skip to main content
. 2015 Dec 2;147(1):114–124. doi: 10.1111/imm.12545

Figure 1.

Figure 1

Colonic neutrophil recruitment and neutrophil chemokine expression in response to Clostridium difficile infection in the absence of interleukin‐23 (IL‐23) signalling. (a) Analysis of CD11b and Ly6G expression profiles of CD45+ colonic leucocytes. The number in bold type represents the percentage of total CD45+ leucocytes contained within the indicated gate. (b) Total number of recruited CD11bHigh Ly6GH igh neutrophils as defined in (a). Bars represent mean ± SEM number of recruited neutrophils for the indicated group. n = 8 per group. For all animals, the entire colon was taken for FACS analysis. (c) Colonic gene expression was assessed using quantitative PCR as outlined in the Materials and methods. n ≥ 6 per group. Data are shown as mean ± SEM fold change gene expression of wild‐type (WT) C. difficile‐infected (black bars) and IL‐23KO C. difficile‐infected (grey bars) animals compared with untreated WT mice. CDI = C. difficile infected. For all analyses, *P < 0·05 compared with untreated WT animals and brackets indicate P < 0·05 for the differences between indicated groups.