FIG 5.

Characterization of proteolytic activities of ΔaprA, ΔlasB and ΔaprA ΔlasB mutants. (A) Qualitative analysis of protease activity using milk plate assays. (B) Qualitative analysis of protease activity of cell-free supernatant samples, treated with LB or BHI for 18 h, using skim milk assays. Incubation of A549-Gluc cells were incubated with with flagellin pretreated cell-free supernatant samples grown in LB (C) or BHI (D) for 18 h. Data are presented as means from six experiments ± SD. Significance was evaluated by Wilcoxon test. *, P < 0.05 relative to results for the PA14 WT control.