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Construction of and purification of lipopeptide SitC18. (A) pCtuf-SitC18 is the elongation expression plasmid for the truncated SitC protein gene consisting of a signal peptide and 10 amino acids. The 3′ end is extended by a Strep tag sequence and a stop codon. (B) Amino acid sequence of SitC18. (C) SDS-PAGE with purified SitC-18.
