FIG 6.
Stimulation of HEK293 and HEK293-TLR2 cells by USA300 JE2 and its Δlgt mutant cultured in TSB with and without supplemented 50 μM FFAs. HEK cells were stimulated with 106 bacteria. USA300 JE2 and the Δlgt mutant were cultured in TSB medium alone or supplemented with 50 μM UFFAs (C16:1, C18:1, C18:2, C16:0, and C18:0) for 17 h. IL-8 was measured after 18 h of stimulation. The experiments in duplicate were conducted at least 3 times. Error bars indicate SEMs. Statistical significances were calculated to compare test groups with the control (TSB) by using unpaired Student's t test. ns, not significant (P > 0.05); ***, P < 0.001.