FIG 1.

Essential role of C3 and C5 in phagocytosis and killing of C. neoformans by neutrophils. (A) Immunofluorescent confocal images showing C. neoformans (green) inside neutrophils (red) incubated in the presence of wild-type (WT) mouse serum (left) or outside neutrophils incubated with C3^−/− mouse serum (right). (B) Percentage of yeast cells ingested by neutrophils in the presence of wild-type, C3^−/−, or C5^−/− mouse serum or wild-type mouse serum with cytochalasin D (Cyto D). (C) Percentage of yeast cells killed by neutrophils in the presence of wild-type, C3^−/−, C5^−/−, or heat-inactivated (HI) wild-type mouse serum. (D) Percentage of yeast cells killed by neutrophils in the presence of wild-type mouse serum and cytochalasin D. (E) Percentage of yeast cells killed by neutrophils in the presence of wild-type mouse serum and diphenyleneiodonium (DPI). Data are presented as means ± standard errors of the means. Data are representative of results from 3 independent experiments. **, P < 0.01; ***, P < 0.001.