FIG 3.

Cleavage of Prdx6 by Pla disrupts both phospholipase A₂ and peroxidase activities. (A) Peroxidase activity of Prdx6 following incubation with Y. pestis, Y. pestis Pla D206A, or trypsin or incubation alone for 2 h at 37°C. Prdx6 activity is calculated as the percentage of H₂O₂ removed, based on the ratio of the amount of H₂O₂ removed in the presence to that removed in the absence of Prdx6. (B) Phospholipase A₂ activity of Prdx6 following incubation with Y. pestis, Y. pestis Pla D206A, or the inhibitor MJ33 or incubation alone. One representative experiment of 2 biological replicates is shown, and error bars represent the SEM (*, P < 0.05; **, P < 0.01; ***, P < 0.001 by one-way ANOVA with Bonferroni's multiple-comparison test).