FIG 4.

Pla-dependent changes in Prdx6 levels within the lung during Y. pestis respiratory infection. Abundance of extracellular Prdx6 recovered by BAL of C57BL/6 mouse lungs 48 h post-inoculation with PBS (mock), 10⁴ CFU of Y. pestis, 10⁴ CFU of Y. pestis Δpla, or 10⁸ CFU of Y. pestis Δpla as measured by ELISA (A) or immunoblotting (B). Data from mock- or wild-type Y. pestis-infected C57BL/6 prdx6^−/− mice are shown as controls in panel B. The input CFU and output CFU are included below the graph and blot to denote the number of bacteria inoculated and present at the time of assessment. The density of each band relative to BALF is indicated beneath the immunoblot. Data in panel A are combined from 2 independent experiments (n = 10), and error bars represent the SEM. Data in panel B are representative of 3 independent experiments (*, P < 0.05; ns, not significant by one-way ANOVA with Bonferroni's multiple-comparison test).