FIG 4.

A conserved tissue-independent mechanism equilibrates the ratio of antigen-experienced CD4⁺ IFN-γ–YFP⁺ and CD4⁺ IFN-γ–YFP⁺ IL-10–GFP⁺ populations during malaria infection. IFN-γ and IL-10 dual-reporter mice were infected (i.v.) with 1 × 10⁴ P. yoelii NL pRBCs. (A) The frequencies of antigen-experienced CD4⁺ T cells in the different tissues that express IFN-γ–YFP during the course of infection. (B) The proportion of IFN-γ–YFP⁺ parasite-specific CD4⁺ T cells that coexpressed IL-10–GFP in the different tissues over the course of infection. The results are the mean ± SEM for the group from 4 to 5 independent experiments (3 to 5 mice in each experiment). **, P < 0.05 between the Peyer's patches group and all other groups (at the corresponding time point); ¶, P < 0.05 between the liver group and all other groups (at the corresponding time point). Significance was tested using two-way ANOVA with Tukey post hoc analysis.