TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| V. vulnificus | ||
| CMCP6 | Wild type, clinical isolate | CNU Hospital |
| ΔtonB1 | CMCP6 with in-frame deletion of entire structural genes in TonB1 locus | This study |
| ΔtonB2 | CMCP6 with in-frame deletion of entire structural genes in TonB2 locus | This study |
| ΔtonB3 | CMCP6 with in-frame deletion of entire structural genes in TonB3 locus | This study |
| ΔtonB12 | CMCP6 with in-frame double deletion of entire structural genes in TonB12 loci | This study |
| ΔtonB13 | CMCP6 with in-frame double deletion of entire structural genes in TonB13 loci | This study |
| ΔtonB23 | CMCP6 with in-frame double deletion of entire structural genes in TonB23 loci | This study |
| ΔtonB123 | CMCP6 with in-frame triple deletion of entire structural genes in TonB123 loci | This study |
| E. coli | ||
| DH5α | F− recA1 restriction negative | Laboratory collection |
| SY327 λ pir | Δ(lac pro) argE(Am) rif nalA recA56 λ pir lysogen | 26 |
| SM10 λ pir | thi thr leu tonA lacY supE recA::RP4-2-Tcr::Mu Kmr λ pir lysogen | 26 |
| Plasmids | ||
| pDM4 | Suicide vector with ori R6K sacB; Cmr | 58 |
| pLAFR3 | IncP cosmid vector; Tcr | 59 |
| pRK2013 | IncP Kmr Tra Rk2+ repRK2 repE1 | 60 |
| pCMM1509 | pDM4 with a 2-kb SacI/SpeI in-frame deleted tonB1 operon | This study |
| pCMM1510 | pDM4 with a 2-kb SacI/SpeI in-frame deleted tonB2 operon | This study |
| pCMM1511 | pDM4 with a 1.9-kb SacI/SmaI in-frame deleted tonB3 operon | This study |
| pCMM1512 | pLAFR3 with a 25-kb fragment containing the in-frame deleted tonB1 operon | This study |
| pCMM1513 | pLAFR3 with a 25-kb fragment containing the in-frame deleted tonB2 operon | This study |
| pCMM1514 | pLAFR3 with a 25-kb fragment containing the in-frame deleted tonB3 operon | This study |
a
Cmr, Cm resistance; Tcr, Tc resistance; Apr, Ap resistance; Kmr, Km resistance.