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PorU protein is not localized to the membrane fractions of the Δomp17 strain. Whole-cell lysates (A) and the cytoplasm-plus-periplasm and membrane fractions (B) of P. gingivalis ATCC 33277 and the Δomp17 strain were subjected to SDS-PAGE and immunoblot analysis with anti-PorU. M, marker; CP/PP, cytoplasm plus periplasm; TM, total membrane; CBB, Coomassie brilliant blue staining.
