FIG 1.

EscE is secreted and translocated in a T3SS-dependent manner. (A) EscE is secreted into the culture supernatant in a T3SS-dependent manner. Five percent of the TBP and 10% of the ECP from similar amounts of bacteria grown in DMEM were separated using Tricine–SDS-PAGE and transferred onto PVDF membranes for immunoblotting. (B) EscE is translocated into J774A.1 cells. J774A.1 cells were infected with E. tarda strains carrying the plasmid pACYC-eseG::cyaA or pACYC-escE::cyaA, and intracellular cAMP levels were determined at 5 h postinfection. Means and SD from the results of one representative experiment are shown. ***, P < 0.001; NS, not significant.