TABLE 1.
Strains and plasmid vectors used in this study
| Strain or plasmid | Description or relevant characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| E. tarda | ||
| PPD130/91 | Wild type; Kms Colr Amps; LD50 = 105.0 | 17 |
| ΔescE | PPD130/91 with in-frame deletion of escE | This study |
| ΔesaN | PPD130/91 with in-frame deletion of esaN | 14 |
| wt/pACYC-escE-2HA | PPD130/91 with pACYC-escE-2HA | This study |
| ΔesaN/pACYC-escE-2HA | ΔesaN with pACYC-escE-2HA | This study |
| ΔescE/pACYC-escE-2HA | ΔescE with pACYC-escE-2HA | This study |
| ΔescE/pACYC-escEΔ2–15-2HA | ΔescE with pACYC-escEΔ2–15-2HA | This study |
| ΔescE/pACYC-gst-2HA | ΔescE with pACYC-gst-2HA | This study |
| ΔescE/pACYC-gst::escE-2HA | ΔescE with pACYC-gst::escE-2HA | This study |
| wt/pACYC-eseG::cyaA | PPD130/91 with pACYC-eseG::cyaA | 13 |
| ΔesaN/pACYC-eseG::cyaA | ΔesaN with pACYC-eseG::cyaA | 13 |
| wt/pACYC-escE::cyaA | PPD130/91 with pACYC-escE::cyaA | This study |
| ΔesaN/pACYC-escE::cyaA | ΔesaN with pACYC-escE::cyaA | This study |
| ΔescE/pJN105-gst::escE-2HA | ΔescE with pJN105-gst::escE-2HA | This study |
| ΔescE/pJN105-gst::escE-2HA/pACYC-eseG::cya | ΔescE with pJN105-gst::escE-2HA and pACYC-eseG::cyaA | This study |
| E. coli | ||
| DH5α | α complementation; Amps | Stratagene |
| S17-1 λpir | RK2 tra regulon; pir; host for pir-dependent plasmid | 19 |
| Plasmids | ||
| pMD18-T | Cloning vector; Ampr | TaKaRa |
| pRE112 | pGP704 suicide plasmid; pir dependent; Cmr oriT oriV sacB | 18 |
| pACYC-184 | Tetr Cmr | Amersham |
| pJN105 | Arabinose-inducible gene expression vector; araC-PBAD; Gmr | 20 |
| pRE112-escE | pRE112 containing escE flanking fragments with aa 2 to 74 deleted | This study |
| pACYC-escE-2HA | pACYC184 with escE-2HA; Tetr | This study |
| pACYC-escEΔ2–15-2HA | pACYC184 with escE lacking 2nd to 15th amino acid; Tetr | This study |
| pACYC-escE::cyaA | pACYC184 with cyaA fused to C terminus of escE; Cmr | This study |
| pACYC-eseG::cyaA | pACYC184 with cyaA fused to C terminus of full-length eseG gene; Cmr | 13 |
| pJN105-gst::escE-2HA | pJN105 with gst::escE-2HA; Gmr | This study |
| pACYC-gst-2HA | pACYC184 with gst-2HA; Tetr | This study |
| pACYC-gst::escE-2HA | pACYC184 with gst fused to N terminus of escE-2HA; Tetr | This study |
a
Km, kanamycin; Col, colistin; Amp, ampicillin; Cm, chloramphenicol; Gm, gentamicin; Tet, tetracycline.