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Number of pneumococci recovered from blood (A), the lungs (B), and nasopharynx (C) after intranasal infection. Note that ΔSPD0247Comp was tested only in the blood. Each mouse received approximately 5 × 105 CFU/ml. For each time point, 10 mice for ΔSPD0247 (■) and ΔSPD0247Comp (◆) and 15 to 20 mice for D39 (●) were used. Vertical bars indicate the standard errors of the means. *, P < 0.05; **, P < 0.01 (significant difference between the ΔSPD0247 mutant and D39).
