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B. abortus infection reduced Cx43 expression in osteocytes. (A) Relative expression of the Cx43 gene was assessed by qRT-PCR in noninfected (NI) and B. abortus (Ba)-infected MLO-Y4 cells. (B) Cx43 was revealed by immunofluorescence with a specific antibody at 48 h postinfection. Data are given as the means ± SD from at least three individual experiments. Data shown are from a representative experiment of three performed. ***, P < 0.001, versus results in noninfected cells.
