FIG 6.

B. abortus virB10 mutant does not induce TNF-α secretion by osteocytes. Levels of TNF-α (A and B), RANKL (C and D), and IL-6 (E and F) were measured in culture supernatants from noninfected (NI) and B. abortus (Ba)-infected or B. abortus virB10 mutant (ΔvirB10)-infected MLO-Y4 cells by ELISA at 24 and 48 h postinfection. PMA, phorbol myristate acetate. Data are given as the means ± SD from at least three individual experiments. *, P < 0.1; **, P < 0.01; ***, P < 0.001, versus results in noninfected cells.