FIG 2.

DksA is required for S. Typhimurium motility. S. Typhimurium SL1344, its corresponding dksA mutant strain, and the dksA strain harboring the plasmid pWSK29 or pWSK29::dksA were grown in LB for 16 h. A 10-μl portion of each culture was placed in the middle of 0.3% agar LB or M9 plates. LB plates were incubated at 37°C for 5 h (A), and M9 plates were incubated for 21 h (B). Bars show the mean motility (from three independent plates) relative to that of the wild-type strain. One-way analysis of variance (ANOVA) with Dunnett's multiple-comparison test was implemented to compare the motility of the different strains with the wild-type motility. ***, P < 0.0001; ns, not significant. (C) Representative swimming plates. The motility halo of each strain on M9 plates was imaged by a Pentax K-3 II digital SLR camera.